Our laboratory is seeking a research assistant to study the adaptive immune system in autoimmune diseases. We use small animal (mouse) models of type 1 diabetes and rheumatoid arthritis to study why the immune system generates "friendly fire" and attacks the body's own tissues. Laboratory duties include DNA preparation and polymerase chain reaction (PCR) to identify and characterize target molecules. Opportunity exists for cloning and expressing these molecules for in vitro studies. Antibody assays with ELISA are used to measure autoantibody production and ELIspot assays are used to identify cytokines and other molecules produced by lymphocytes. Flow cytometry and mass cytometry are used to examine subset of T and B lymphocytes. These state of the art techniques are used to identify early biomarkers that can help predict early stages of diabetes and arthritis before the disease is fully developed. This is a great opportunity to grow scientific skill sets necessary for modern molecular biology and immunology.
Standard procedures plus:
Counting and staining cells and data analysis for flow cytometry.
Counting and staining cells for microscopy.
Enzyme linked immunoassay for antibodies.
Tissue culture techniques.
Small animal (mouse) dissection.
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* American Association for the Advancement of Science: The School of Medicine has 112 elected fellows
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